VIASURE Salmonella Real Time PCR Detection Kit is designed for specific identification of Salmonella in human stool samples from patients with signs and symptoms of Salmonella infection (salmonellosis).
This test is intended for use as an aid in the diagnosis of Salmonella infection in humans in combination with clinical and epidemiological risk factors.
DNA is extracted from stool specimens, multiplied using Real Time amplification and detected using specific primers and a fluorescent reporter dye probe for Salmonella.
Salmonella species are a leading bacterial cause of acute gastroenteritis with major impact on public health.
The Salmonella genus is divided taxonomically into six Salmonella enterica subspecies and Salmonella bongori. Up to date, more than 2,500 Salmonella serovars are described which are pathogenic for humans. Salmonella causes two types of diseases: enteric (typhoid) fever and acute gastroenteritis, commonly referred to as salmonellosis.
Transmission of Salmonella occurs through contaminated food (poultry and poultry products, beef, pork, eggs, milk, and seafood), water or contact with infected animals. Patients infected with Salmonella frequently suffer nausea, vomiting, abdominal cramps/pains, achiness, fever, intense diarrhea and/or headache.
Target gene most frequently employed for the specific detection of Salmonella is the virulence invA gene (Salmonella invasion protein gene), which is located on the Salmonella pathogenicity island 1.