VIASURE Real Time PCR Detection Kits

Flu Typing II (H1N1, H5N1, H3N2 & H7N9)


VIASURE Flu Typing II Real Time PCR Detection Kit is designed for specific identification and differentiation of Influenza A (H1N1)pdm09, H3N2, H5N1, and H7N9 subtypes in respiratory samples from patients with signs and symptoms of respiratory infection.

This test is intended for use as an aid in the diagnosis of Influenza A (H1N1)pdm09, H3N2, H5N1, and H7N9 subtypes in combination with clinical and epidemiological risk factors.

RNA is extracted from specimens, amplified using RT-PCR and detected using fluorescent reporter dye probes specific for Influenza A(H1N1)pdm09, H3N2, H5N1, and H7N9 subtypes.


Real Time PCR
Specimen Type
Respiratory samples
Storage up to
(From production) 2 years
Storage temperature
(Also transport) Room temperature


Influenza virus is an enveloped, single stranded RNA virus that belongs to the Orthomyxoviridae family and causes the majority of viral lower respiratory tract infections. Influenza A and B are a significant cause of morbidity and mortality worldwide, considering that elderly and compromised individuals are especially at risk of developing severe illness and complications such as pneumonia. People feel some or all of these symptoms: fever or feeling feverish/chills, cough, sore throat, nasal stuffiness and discharge, myalgia, headaches, and anorexia.

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The influenza viruses can be spread from person to person in two different ways: through the air (large droplets and aerosols from sneezing and coughing), and by direct or indirect contact.

Influenza A viruses has been classified into subtypes based on the combinations of the envelope proteins hemagglutinin (HA) and neuraminidase (NA). Currently, influenza A(H1N1) and A(H3N2) are the circulating seasonal influenza A virus subtypes. This seasonal A(H1N1) virus is the same virus that caused the 2009 influenza pandemic. Influenza A(H3N2v) viruses were first detected in people in 2011, being its infections associated with prolonged exposure to pigs at agricultural fairs. Besides, humans can also be infected with avian influenza virus subtypes A(H5N1) and A(H7N9) since their emergence in China in 2003 and 2013, respectively. Influenza A(H5N1) virus has spread from Asia to Europe and Africa, and has become entrenched in poultry populations in some countries. Outbreaks have resulted in millions of poultry infections and several hundred human cases, who often display severe pneumonia and greater than 50% mortality. Avian Influenza A(H7N9) virus spreads faster than H5N1 and commonly resulted in severe respiratory illness, although its mortality rate (20%) is lower than that attributed to H5N1 virus.

Diagnosis can be problematic, as a wide range of clinical presentation of influenza. Real-time PCR assays have been shown to be a sensitive and specific diagnostic tool for the Influenza A subtyping.

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