VIASURE Adenovirus Real Time PCR Detection Kit is designed for specific identification of Adenovirus in human stool samples from patients with signs and symptoms of gastrointestinal infection.
This test is intended for use as an aid in the diagnosis of Adenovirus infection in humans in combination with clinical and epidemiological risk factors.
DNA is extracted from stool specimens, multiplied using Real Time amplification and detected using specific primers and a fluorescent reporter dye probe for Adenovirus.
Adenoviruses belong to the Adenoviridae family of non-enveloped and double-stranded (dsDNA) viruses.
There are 51 immunologically distinct human Adenovirus serotypes classified into 6 species (HAdV-A to HAdV-F) that can cause human infections ranging from respiratory disease (mainly species HAdV-B and C), and conjunctivitis (HAdV-B and D), to gastroenteritis (HAdV-F serotypes 40 and 41). In addition to the previous serotypes, the HAdV 31, 12, 18, 1, 2, 5 and 6 have been associated with acute diarrhea.
Although epidemiologic characteristics of the Adenovirus vary by type, all are transmitted by direct contact or by fecal-oral route. They are pathogenic for all age groups of the population, especially for children. These viruses are highly infectious; few viral particles are sufficient to get sick.
Target gene most frequently employed for the specific detection of HAdV is the hexon gene.