VIASURE Real Time PCR Detection Kits
VIASURE Adenovirus, Metapneumovirus & Bocavirus Real Time PCR Detection Kit is designed for specific identification and differentiation of human Adenovirus, Metapneumovirus and Bocavirus in respiratory samples from patients with signs and symptoms of respiratory infection.
This test is intended for use as an aid in the diagnosis of Adenovirus, Metapneumovirus and/or Bocavirus in combination with clinical and epidemiological risk factors.
RNA/DNA is extracted from clinical specimens, multiplied using Real Time amplification and detected using specific primers and a fluorescent reporter dye probe for Adenovirus, Metapneumovirus and Bocavirus.
Adenoviruses belong to the Adenoviridae family of non-enveloped and double-stranded (dsDNA) viruses. There are more than 50 immunologically distinct human Adenovirus serotypes classified into 6 species (Adenovirus-A to Adenovirus-F) that can cause human infections ranging from respiratory disease (mainly species Adenovirus-B and C), and conjunctivitis (Adenovirus-B and D), to gastroenteritis (Adenovirus-F serotypes 40 and 41). Adenoviruses can be spread due to close personal contact or air transmission by coughing and sneezing. Manifestations of respiratory infections include common cold, fever, sore throat (pharyngitis), bronchitis and pneumonia.
Human Metapneumoviruses, belong to the Paramyxoviridae family and are an important cause of upper and lower respiratory infection. Metapneumovirus is an enveloped, single-stranded, negative-sense RNA virus. Clinical symptoms of Metapneumovirus include cough, fever, nasal congestion, and shortness of breath, and may progress to bronchiolitis or pneumonia. Metapneumovirus is mainly transmitted by infectious airborne droplets, and it has been reported as the second most frequently found virus in respiratory infections, with children younger than five years being the most susceptible to infection.
Human Bocaviruses belong to the Parvoviridae family and are believed to be important causative agents of upper and lower respiratory tract infections in young children. Bocaviruses are small non-enveloped single-stranded DNA viruses that contain a genome of about 5.3 kb. Bocaviruses are highly prevalent in co-infections with other pathogens. Clinical manifestations range from mild upper respiratory tract infections to bronchiolitis, and lower respiratory tract diseases, such as pneumonia. The common symptoms are fever, cough, acute otitis media, tonsillitis and conjunctivitis, sinusitis, and rhinorrhea.
Diagnosis can be problematic, as a wide range of pathogens can cause acute respiratory infections presenting with similar clinical syndromes. Real-time PCR assays have been shown to be a sensitive and specific diagnostic tool for the detection of Adenovirus, Metapneumovirus and Bocavirus.