For Research Use Only (RUO). Not for use in diagnostic procedures.

VIASURE HLA-B*27 Real Time PCR Detection Reagents RUO is a non-automatised real-time PCR test designed for the qualitative detection of all the currently known HLA-B*27 human alleles in peripheral whole blood and buccal swab samples.
After DNA isolation, the identification of HLA-B*27 is performed by the amplification of highly homologous regions of the HLA-B*27 allele group, using specific primers and fluorescent-labelled probes.a multiplex real-time PCR designed to detect Y-chromosome specific genes in fetal free circulating DNA isolated from maternal plasma (cell-free fetal DNA, or cffDNA). The isolated DNA is amplified on specific regions corresponding to the Y-chromosome specific genes DYS14, SRY and DAZ using specific primers and a fluorescent-labeled probes.

The assay uses a human housekeeping gene as an Endogenous Internal Control (EIC) (human β-globin (HBB) gene) to follow-up the integrity of the sample, monitor the extraction process, discard the inhibition of the polymerase activity and/or verify the correct functioning of the amplification mix.