Raw Materials for molecular diagnostic
High quality solutions for developing and manufacturing your Molecular Diagnostic IVD assays.
Optimized molecular biology enzymes for qPCR applications (DNA Taq polymerase and Reverse Transcriptase) and anti-Taq DNA polymerase monoclonal antibodies (qPCR hot start system) for your qPCR assays.
- Taq DNA Polimerase
- Retrotranscriptase
- Klenow Taq DNA
- Ligase
- Anti-Taq DNA Polimerase mAb
- Lyophilized molecular biology enzymes kits
Optimized real time qPCR Master mixes. Choose the most suitable Master mix, liquid or lyophilized for your DNA or RNA qPCR assays.
- Liquid Master Mix for DNA & RNA Detection
- Liquid Master Mix to Be Lyophilized for DNA & RNA Detection
- Lyophilized Master Mix for DNA & RNA Detection (50 & 1000 Reactions)
Prevalent range of length is 12 mer up to 200 mer, purification method desalting or HPLC and any production scale could be provided.
We also provide single stranded RNA substituted in C2 by -H or -OMe. We synthesize oligonucleotides using high quality CPG support and amidites from world leading suppliers.
- ssDNA Unsubstituted
- ssRNA Substituted in C2
Double stranded DNA, suitable as positive controls for your PCR assays. Products available in liquid & lyophilized format from 100 to 3000 pb. Choose the most suitable one for your RT-qPCR positive controls.
Any quantity (nmol): 50, 200, 1000… Purified by RP-HPLC.
Prevalent range of length: 12 mer up to 50 mer for probes.
- Taqman monoquenched & doublequenched probes
- Biolocked Taqman probes (Locked Nucleid Acids probes)
- Taqman probes with MGB
Most common Dual-Labeled probes for qPCR. During PCR, the target-bound probe gets hydrolized by the enzyme activity realising the fluorophore (FAM, HEX, ROX, TxR and Cy5) and quencher (BHQ, Eclipse, etc).
ROX / Cy5
BHQ-2 / CBQX2 / CBQx3
These probes are usually used for mutant and wild type strains detection but it can be used for other applications. Their bases are substituted by locked nucleic acid bases which increases the Tm.
ROX / TxR / Cy5
BHQ-2 / CBQX2 / CBQX3
These probes end with a polyamine that increases their Tm by binding to the DNA template. This enhances their specificity and allows them to be used in assays to discriminate between different strains of microorganisms.
Molecular diagnostic
Certest Raw Materials provides high quality solutions for developing and manufacturing your Molecular Diagnostic IVD assays.
Our expertise in primers & probes synthesis and purification, molecular biology enzymes manufacturing and dsDNA synthesis offers to our customers
a priceless support in their challenging projects.
All these items are already being used by our Viasure division in all their IVD products with excellent results.
Additionally, we also manufacture our own fluorophores & quenchers that allows us to have the best tools available and the proper knowledge for
manufaturing your customized probes in a competitive time.
Our experience with qPCR diagnosis has helped us to better understand the challenges to provide specific products to specialized customers.
