VIASURE Tick Borne Diseases Real Time PCR Detection Kit is designed for the specific identification and differentiation of viral RNA or genomic DNA specific for Tick Borne Encephalitis Virus (TBEV), Rickettsia spp., Babesia microti, Babesia divergens, Ehrlichia chafeensis, Ehrlichia muris, Borrelia burgdorferi sensu lato (s.l.), Borrelia miyamotoi and/or Borrelia hermsii, Anaplasma phagocitophylum and/or Coxiella burnetii in blood, serum, tissue samples and microbiological culture from ticks, biopsy skin, cerebrospinal fluid (CSF) and synovial fluid from patients with signs and symptoms of Tick Borne diseases.
This test is intended for use as an aid in the diagnosis of Tick Borne diseases in combination with clinical and epidemiological risk factors.
RNA/DNA is extracted from clinical specimens, multiplied using Real Time amplification and detected using fluorescent reporter dye probes specific TBEV, Rickettsia spp., Babesia microti, Babesia divergens, Ehrlichia chafeensis, Ehrlichia muris, Borrelia burgdorferi s.l., Anaplasma phagocitophylum and Coxiella burnetii.
Method: Real Time PCR
Specimen Type: Clinical samples
Storage up to: (From production) 2 Years
Storage temperature: (Also transport) Room temperature °C
Tick Borne diseases comprise a group of infections transmitted to humans by the bite of ticks infected with bacteria, viruses, or parasites.
Tick Borne diseases affecting humans include: Lyme disease, relapsing fever, babesiosis, anaplasmosis, ehrlichiosis, Q fever, Tick Borne encephalitis and spotted fever among others.
Lyme disease (or Lyme borreliosis) is the most common Tick Borne disease worldwide. It is caused by the spirochaete Borrelia burgdorferi and it is transmitted to humans through the bite of infected blacklegged ticks of the genus Ixodes. Typical symptoms of Lyme disease include fever, headache, fatigue and a characteristic skin rash called erythema migrans. If left untreated, infection can spread to joints, the heart, and the nervous system.
Borrelia miyamotoi and B. hermsii are species of spiral-shaped bacteria that is closely related to the bacteria that cause tick-borne relapsing fever (TBRF). First identified in 1995 in ticks from Japan, B. miyamotoi has also been detected in different Ixodes ticks species, whereas spirochete Borrelia hermsii is transmitted by its argasid tick vector, Ornithodoros hermsi. Patients with this infection were most likely to have fever, chills, and headache. Other common symptoms included body and joint pain and fatigue.
Anaplasmosis is caused by the bacterium Anaplasma phagocytophilium and is transmitted to humans through ticks of the genus Ixodes. Anaplasmosis shows high clinical variability, with symptoms like headache, fever, chills, malaise, muscle pain, nausea, cough, confusion and rash. If not treated correctly, anaplasmosis can evolve to severe clinical manifestations and even death in <1% of cases. Q fever is a zoonosis caused by the bacteria Coxiella burnetii. Cattle, sheep, and goats are the primary reservoirs of Coxiella burnetii, and transmission to humans occurs primarily through inhalation of aerosols from contaminated soil or animal waste, though it can be transmitted through tick bites. Only about 50% of the infected people show clinical symptoms, which are flu-like symptoms such as headache, fever, chills, fatigue, muscle aches, nausea, cough, chest pain and weight loss. In severe cases people may develop pneumonia or hepatitis.
Babesiosis is produced by many species of protozoa of the genus Babesia, mainly Babesia microti and Babesia divergens. Babesiosis is also transmitted by ticks of the genus Ixodes, and it is frequently found as a coinfection with Lyme disease. Babesiosis usually shows none or flu-like mild symptoms, such as headache, fever, chills, body aches, loss of appetite, nausea or fatigue. However, since Babesia parasites infect and destroy red blood cells, babesiosis can lead to hemolytic anemia.
Ehrlichiosis is due to different species of bacteria of the genus Ehrlichia. Causative agents in humans are Ehrlichia chaffeensis and Ehrlichia muris. Ehrlichiosis is transmitted by the lone star tick (Amblyomma americanum). Typical symptoms of ehrlichiosis include headache, fever, chills, malaise, muscle pain, nausea, conjunctival infection, confusion and rash. Severe disease may present lethargy, myalgia, reduction of sodium levels and platelets and elevated liver enzymes, with fatal outcome in 3% of cases.
Tick Borne encephalitis is produced by the Tick Borne encephalitis virus (TBEV) of the family Flaviviridae. It is transmitted to humans through ticks of the genus Ixodes. The onset of the disease includes nonspecific symptoms such as fever, malaise, anorexia, muscle aches, headache, nausea and vomiting. In 20-30% of patients, a second phase is observed that involves the central nervous system with symptoms of meningitis, encephalitis, or meningoencephalitis, leaving neuropsychiatric sequelae in 10-20% of patients.
Spotted fever is caused by bacteria of the genus Rickettsia and is widely distributed by different geographical areas, being able to transmit depending on it by different ticks such as: wood tick, Dermacentor Andersoni (in the Rocky Mountain states, USA), dog tick, Dermacentor variabilis (other areas of the USA), ticks Amblyomma cajennense (in South America) and Rhipicephalus sanguineus (in Mexico). Spotted fever is characterized by darks scabs at the site of the tick bite (eschar), accompanied by nonspecific symptoms such as headache, fever, rash and muscle ache.
Since most Tick Borne diseases show similar symptoms, diagnosis can be problematic. Real-time PCR assays have been shown to be a sensitive and specific diagnostic tool for the detection of the causative agent.