Molecular Biology Enzymes & Hot Start PCR
Anti-Taq DNA Polimerase mAb
Lyophilized molecular biology enzymes kits
Liquid Master Mix for DNA & RNA Detection
Liquid Master Mix to Be Lyophilized for DNA & RNA Detection
Lyophilized Master Mix for DNA & RNA Detection (50 & 1000 Reactions)
Taqman monoquenched & doublequenched probes
Biolocked Taqman probes (Locked Nucleid Acids probes)
Optimized molecular biology enzymes for qPCR applications (DNA Taq polymerase and Reverse Transcriptase) and anti-Taq DNA polymerase monoclonal antibodies (qPCR hot start system) for your qPCR assays.
Molecular Biology Enzymes & Hot Start PCR
- Taq DNA Polimerase
- Retrotranscriptase
- Klenow Taq DNA
- Ligase
- Anti-Taq DNA Polimerase mAb
- Lyophilized molecular biology enzymes kits
Molecular Biology Enzymes & Hot Start PCR > Taq DNA Polimerase
MT-25TAQ
Thermus aquaticus (Taq) polymerase Glycerol free (50.000 u)
MT-25TAQk
Thermus aquaticus (Taq) polymerase without 5’3’ exonuclease activity Glycerol free (25.000 u)
Molecular Biology Enzymes & Hot Start PCR > Retrotranscriptase
MT-25RT2
Reverse Transcriptase (RT2) Glycerol free (250.000 u)
Molecular Biology Enzymes & Hot Start PCR > Klenow Taq DNA
MT-25T4K
T4 DNA Polynucleotide Kinase
Molecular Biology Enzymes & Hot Start PCR > Ligase
Molecular Biology Enzymes & Hot Start PCR > Anti-Taq DNA Polimerase mAb
MT-16TQ01
Anti-TAQ polymerase mAb (clone TQ01) (x1mg)
MT-16TQ32
Anti-TAQ polymerase mAb (clone TQ32) (x1mg)
Molecular Biology Enzymes & Hot Start PCR > Lyophilized molecular biology enzymes kits
MT-E27RT
Kit Reverse Transcriptase (RT2), lyophilized (50000 U)
MT-E27TAQ
Kit Thermus aquaticus (Taq) polymerase, lyophilized (5000 U)
MT-E27TAQk
Kit Thermus aquaticus (Taq) polymerase without 5’-3’ exonuclease activity, lyophilized (5000 U)
Optimized real time qPCR Master mixes. Choose the most suitable Master mix, liquid or lyophilized for your DNA or RNA qPCR assays.
Master mix
- Liquid Master Mix for DNA & RNA Detection
- Liquid Master Mix to Be Lyophilized for DNA & RNA Detection
- Lyophilized Master Mix for DNA & RNA Detection (50 & 1000 Reactions)
Master mix > Liquid Master Mix for DNA & RNA Detection
MT-25MXD01
Liquid DNA Master Mix Hot Start (100 reactions)
MT-25MXR01
Liquid RNA Master Mix Hot Start (100 reactions)
Master mix > Liquid Master Mix to Be Lyophilized for DNA & RNA Detection
MT-E25MXD02
Liquid DNA Master Mix Hot Start for lyophilization (200 reactions)
MT-E25MXR02
Liquid RNA Master Mix Hot Start for lyophilization (200 reactions)
Master mix > Lyophilized Master Mix for DNA & RNA Detection (50 & 1000 Reactions)
MT-E27MXD01
Lyophilized DNA Master Mix Hot Start (50 reactions)
MT-E27MXR01
Lyophilized RNA Master Mix Hot Start (50 reactions)
Prevalent range of length is 12 mer up to 200 mer, purification method desalting or HPLC and any production scale could be provided.
We also provide single stranded RNA substituted in C2 by -H or -OMe. We synthesize oligonucleotides using high quality CPG support and amidites from world leading suppliers.
Primers
- ssDNA Unsubstituted
- ssRNA Substituted in C2
Primers > ssDNA Unsubstituted
Code
Length
Purification Methods
Quantity (nmol)
OL-PRIMER
(12-50 mer)
Desalted / RP-HPLC
50 / 200 / 1000
OL-ULDNA
(50-200 mer)
RP-HPLC
50 / 200 / 1000
Primers > ssRNA Substituted in C2
Code
Length
Purification Methods
Quantity (nmol)
OL-MRNA
(12-50 mer)
RP-HPLC
50 / 200 / 1000
Double stranded DNA, suitable as positive controls for your PCR assays. Products available in liquid & lyophilized format from 100 to 3000 pb. Choose the most suitable one for your RT-qPCR positive controls.
dsDNA blocks
Code
Product / Producto
Length
OL-DSDNA
Liquid dsDNA blocks
(100-3000 pb)
OL-DSDNA-L
Lyophilized dsDNA blocks
(100-3000 pb)
Any quantity (nmol): 50, 200, 1000… Purified by RP-HPLC.
Prevalent range of length: 12 mer up to 50 mer for probes.
Probes
- Taqman monoquenched & doublequenched probes
- Biolocked Taqman probes (Locked Nucleid Acids probes)
- Taqman probes with MGB
Most common Dual-Labeled probes for qPCR. During PCR, the target-bound probe gets hydrolized by the enzyme activity realising the fluorophore (FAM, HEX, ROX, TxR and Cy5) and quencher (BHQ, Eclipse, etc).
Probes > Taqman monoquenched & doublequenched probes
Code
Modification 5'
Modification 3'
OL-PROBE
FAM / HEX
ROX / Cy5
BHQ-1 / CBQX
BHQ-2 / CBQX2 / CBQx3
These probes are usually used for mutant and wild type strains detection but it can be used for other applications. Their bases are substituted by locked nucleic acid bases which increases the Tm.
Probes > Biolocked Taqman probes (Locked Nucleid Acids probes)
Code
Modification 5'
Modification 3'
OL-BLPROBE
FAM / HEX
ROX / TxR / Cy5
BHQ-1 / CBX
BHQ-2 / CBQX2 / CBQX3
These probes end with a polyamine that increases their Tm by binding to the DNA template. This enhances their specificity and allows them to be used in assays to discriminate between different strains of microorganisms.
Probes > Taqman probes with MGB
Code
Modification 5'
Modification 3'
OL-MGBPROBE
FAM / HEX / ROX / TxR / Cy5
Eclipse / BHQ1
